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KMID : 0613820130230111365
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Journal of Life Science
2013 Volume.23 No. 11 p.1365 ~ p.1370
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Hydrolysis of Cellulose by Immobilized Cellulase in a Packed Bed Reactor
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Kang Byung-Chul
Lee Jong-Baek
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Abstract
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Immobilized cellulase on weak ion exchange resin showed a typical Langmuir adsorption isotherm. Immobilized cellulase had better stability with respect to pH and temperature than free cellulase. Kinetics of thermal inactivation on free and immobilized cellulase followed first order rate, and immobilized cellulase had a longer half-life than free cellulase. The initial rate method was used to characterize the kinetic parameters of free and immobilized enzyme. The Michaelis-Menten constant K_m was higher for the immobilized enzyme than it was for the free enzyme. The effect of the recirculation rate on cellulose degradation was studied in a recycling packed-bed reactor. In a continuous packed-bed reactor, the increasing flow rate of cellulose decreased the conversion efficiency of cellulose at different input lactose concentrations. Continuous operation for five days was conducted to investigate the stability of long term operation. The retained activity of the immobilized enzymes was 48% after seven days of operation.
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KEYWORD
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Packed-bed reactor, immobilized cellulase, cellulose degradation, Duolite A568
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